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Figure 4 qRT-PCR confirms altered expression of few yield-related genes under abiotic stress conditions.
The pre-treatment measure confirms altered cortical information processing in migraine patients.
However, fluorescence anisotropy measurements revealed an ImportinβDA titration curve that confirms altered binding as compared to Importinβ WT, as e.g., due to an increase in koff.
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Post CO2 sequestration NMR and MICP analysis also confirmed altered pore character in the storage samples.
Western blotting was used to further confirm altered SERPINB2 expression in the toxicant-exposed stem cells (Fig. 3c).
Nevertheless, expression patterns obtained via qRT-PCR confirmed altered expression of these genes under various abiotic stress conditions.
To some extent this has already been investigated but without confirming altered amygdala activation in alexithymia [17], [45], [46].
2-D immunoblotting with highly specific primary antibodies was employed to confirm altered expression levels of distinct quadriceps muscle proteins.
Real-time RT-PCR analysis confirmed altered expression of p53 targets such as p21CIP1 and Pltp, and down-regulation of p21CIP1 downstream genes such as Ube2c, Ccdc99 and Tubb3 in FHL2−/− cells (Fig. 4C).
Additionally, these assays confirmed altered B lymphopoiesis in the collagen X Tg and KO mice, with dramatic reduction in pre-B cell colony outgrowth from the perinatal lethal subset, all of which supports the B220+, CD138+, IgD+, and IgM+ flow cytometry from marrow derived lymphocytes (Figs. 2B,C, 5D) [7], [7].
Further, flow cytometry confirmed altered B lymphopoiesis, which revealed diminished B220+, CD138+, IgM+, and IgD+ lymphocytes from marrows of all outbred and congenic collagen X Tg and KO mice throughout life, with most dramatic reductions in the perinatal lethal mice (Fig. 2B and [5]).
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