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Immunoblotting with an anti-β3 antibody revealed that wild-type αIIbβ3 coimmunoprecipitated with GFP-skeC2, but not with GFP (data not shown), confirming the specifics of interaction of αIIbβ3 with skeC2.
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Mike McCormack, the president of the Toronto Police Association, which represents officers, confirmed that two cops from 13 Division are being investigated by the professional standards unit but said he could not confirm the specifics of the investigation.
Nevertheless, the apoptotic effect can be partially abolished by coincubation with farnesol or geranylgeraniol confirming the specific effect of pamidronate on inhibition of prenylation.
Pretreatment of cell extracts with heat abolished the laddering patterns, confirming the specific detection of telomerase activity.
As a control, we used an antisense probe, which showed no defined localisation, confirming the specific association of the MID1 mRNA with the microtubule-organising centre (Fig. 5c).
No Dnmt2-dependent methylation was detected at C38 of tRNA-Leu, tRNA-Glu, tRNA-ValCAC, and tRNA-His (Appendix Fig S1), thus confirming the specific activity of Dnmt2.
Western blot analysis confirmed the specific immunoprecipitation of several of these proteins (see Figures 4B 4D).
The ability of μB-cAbGFP4 to recognize eGFP was tested as proof-of-principle by fluorescent microscopy and confirmed the specific binding of eGFP to μB-cAbGFP4.
DA analysis of FISH data also confirmed the specific composition of bacterial populations in remission, which is different from control and forms a separate cluster.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com