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ITs did not bind uninfected H9 or 293 T cells, confirming the specific cell binding of ITs.
Also, replacing RGDS with the non-bioactive peptide RGES did not promote a synergistic effect, confirming the specific bioactive role of the peptide and demonstrating that both RGDS and PHSRN signals are required.
However, the activation of the excitatory neurons in the ipsilateral PL, contralateral CG1 or IL did not affect anxiety-like behaviours or heat hyperalgesia (Supplementary Figs 6 8), confirming the specific role of the contralateral PL.
The control viruses caused minimal to mild pathological changes which were comparable between the ADV and ADV-TVA groups, reflecting the inevitable host immune response against adenoviral vector transduction; however, ADV-Emuc-TVA induced distinct and much severer pathological changes compared with the control groups, further confirming the specific pathogenicity of Emuc in vivo (Figs. 1J and S5).
Cold competition with excess amounts of an unlabeled wild-type probe, but not the mutated probe, suppressed the complex formation, confirming the specific binding to the C/EBPβ motif.
Conditioned medium containing wildtype active caspase-1 was capable of inducing apoptosis but the mutant form was not, confirming the specific role of active caspase-1 in inducing cell death (Figure 4B).
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Spicer confirmed the specific date on Tuesday.
The co-immunoprecipitation experiments were repeated with TOMM22 and PEX19 to further confirm the specific interaction.
UV-CLIP assay further confirmed the specific interactions of LoNA and NCL (Supplementary Fig. 3e).
These results confirmed the specific adsorption of anions during the Cu deposition.
All the results confirm the specific advantages of supercritical CO2 towards conventional solvents for filler modification.
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