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Upstream the transcription start sites of 101 sRNA candidates the σ70 consensus promoter motif CTTGAC-N17-CTATAT [ 41] was predicted [Additional file 2], further confirming the identified primary 5'-ends of these sRNAs.
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(c) Amplicon sequencing of FACS-sorted cell fractions confirmed the identified mutations.
Furthermore, optical investigation and SEM images of the tested samples and fracture surfaces confirmed the identified mechanisms evaluated by acoustic emission analysis.
BMI was positively correlated with colpotomy time (.320 and.311, both p = .001), and the generalized linear model confirmed the identified correlation and proved that it was independent from the other variables (Table 4).
Mutagenesis studies were used to confirm the identified epitope.
Both fibroblast and iPS cells were analyzed to re-confirm the identified mutation.
In step II, the user is asked to confirm the identified genes and alternative promoters (Figure 1B).
(5) Checking for SECIS elements was finally conducted to confirm the identified selenoprotein genes.
Western blot and immunohistochemistry were used to confirm the identified genes that were differentially expressed.
In addition to proteomics analysis, we also confirmed the identified signalling proteins at mRNA and protein levels.
Similarly, to confirm the identified targets as generic, we are now carrying out studies in a range of bacterial pathogens.
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CEO of Professional Science Editing for Scientists @ prosciediting.com