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Interestingly, a recent study showed covalent bond formation between a Cpa thioester domain and the small molecule nucleophile spermidine, confirming the accessibility and reactivity of the thioester (Linke-Winnebeck et al., 2014).
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In the present study of xenograft MDA MB, 468 PET imaging with 124I-labeled B-B4 confirmed the accessibility of the CD138 antigen and showed a good stability of tumor activity up to 8 days post injection.
These data independently confirm the accessibility of fused epitopes on the surface of AP205 VLP.
Control hybridizations with the heart-specific markers Nkx2-5 and GATA4 confirmed the accessibility of the heart for hybridization probes (Figure 1).
The binding of SA-gold to biotin-RGDS-conjugated nanofibers confirmed the accessibility of the NCL-conjugated peptides on the nanofiber surface.
Once the production of pure modified proteins by mass spectrometry was confirmed, the accessibility of the reactive azide moieties was established by exploration of a CuAAC reaction with an alkyne-containing rhodamine B fluorescent dye (1) (see SI).
In the enzyme, the Hsred (super-reduced) state is implicated in catalysis,[ 25] and while 4− is not a close model for Hsred it is notable that we are able to confirm the accessibility of discrete FeIFeI hydrides.
Flow cytometry of HA tag-positive cells confirmed the accessibility of scFvE2/p17 at the surface of nonpermeabilized cells, and the absence of significant amounts of scFvG2/p17 molecules at the cell surface.
In addition to confirming the KE antibody accessibility results obtained with VSV-G-KE, this result also suggests that the observed lack of staining of 89.6 WT and VSV-G-LLP2 in Figure 3A is not due to decreased cell surface gp160 expression, as SAR1 reacted similarly with all Envs.
Wild type AP205 VLP did not inhibit antibody binding, confirming the specificity of the inhibition and thereby the accessibility of the peptides on the surface of the VLPs.
The well known miRanda program employs a two-step algorithm: in the first step, an alignment algorithm is used to align the seed region of the miRNA to the 3′UTRs of the target mRNAs and in the second step, cross-species conservation and target accessibility are used for confirming the target, in addition to the several other aforementioned factors [22,23].
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