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Apart from that, HPLC and MS investigations allowed confirming structure of dcII as 7-α d-glucopyranosyl flavokermesic acid.
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This directly confirms structure improvement by annealing.
RT-PCR of 12 selected assemblies confirmed structure and expression in lamellar tissue.
Many restriction enzymes— XbaI, BamHI, EcoRI, HindIII, KpnI, PstI, SacI, SalI, SphI, and PI- PspI (TOYOBO)—were used to confirm structure of the resultant plasmid (Fig. 3).
We also assembled the plastid genome sequence of the R108 line; eliminated ambiguities by Sanger sequencing of amplicons and confirmed structure by DNA gel blot analyses (Section 3.2).
Elemental and spectral analysis were used to confirm structures of the newly synthesized compounds.
Characterization of the monomers by IR, 1H and 13C NMR, GC and elemental analysis confirmed structures and purities.
A CT scan confirmed structures resembling calcium nodules not exceeding 1 cm in diameter with peribronchovascular distribution, affecting both lungs mainly in the inferior lobes.
These agree well with the standard XRD pattern of Fe3O4 nanoparticles [28], thus confirming the structure.
As expected, the GM3 glycan was digested to Lac, confirming this structure in both samples.
A partial data set was collected at room temperature confirming the structure by unit cell determination.
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