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In each case, transduced T cells bound the VY8/B35 tetramer and exhibited VY8-specific IL-2 production (Fig. 1a), confirming specificity and functionality.
SLC38A2 and β-actin mRNA expression was unaffected following transfection of cells with 100 nM SNAT1-specific siRNA (Fig. 2B and C) confirming specificity of the siRNA for its target.
In the case of the complete absence of mRNA (carcinoma No 1), the p16ink4a-specific antibodies did not reveal any cytoplasmic staining confirming specificity of the p16ink4a immunoreactivity.
When RGD receptors were blocked with unconjugated RGD, the tumor uptake was significantly reduced, confirming specificity for integrin αvβ3[18].
CD34 message was detected by reverse transcription polymerase chain reaction predominantly in the CD34+ keratinocytes, confirming specificity of the antibody.
As it can be seen on the transverse ON sections, the patients' AAbs bound to oligodendrocyte cell membranes and processes in the same manner as did anti-MOG antibodies (strong yellow color on double-labeling experiment in Fig. 7c, f), confirming specificity of antibodies to myelin protein.
Similar(19)
The optimized assay condition was validated according to ICH guidelines to confirm specificity, linearity, accuracy and precision.
Adequate controls, including vehicle and similarly modified missense or scrambled sequences, are essential to confirm specificity and to exclude toxicity.
Melting curve analysis was used to confirm specificity of amplification.
Controls were included to confirm specificity and validity.
Preliminary experiments confirmed specificity of siRNAs targeting C/EBP-β and C/EBP-α (Figure 5C).
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