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However, mRNA expression of the splice variant Zfhep2 was higher in DA.PVG-Eae23 (p<0.018) compared to DA (Figure 8D), confirming regulation by the Eae23 locus.
Instantaneous biosynthetic rate measurements showed markedly diminished biosynthesis of endogenous frataxin after treatment with DFO (Fig. 3D), further confirming regulation of frataxin expression by iron.
Importantly, these rhythms were maintained after transfer into DD, confirming regulation by the circadian clock as opposed to being directly light driven (Fig. 1A,B; supplementary material Fig. S1).
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The paper also confirms regulation overlaps and the need for the systematic approaches proposed by [45, 47, 55].
We additionally confirmed regulation of some genes by immunohistochemistry and therefore demonstrate good concordance between gene regulation and coded protein.
Gene Ontology analyses of miR-145 responsive genes confirm regulation of genes involved in cell death, cell cycle regulation and cancer.
Luciferase assays confirmed regulation of Notch signalling by Robo.
In each instance, the qRT-PCR confirmed regulation trends observed in the microarray analysis.
Q-PCR analysis confirmed regulation of these genes in all 3 isolates.
To confirm regulation of four selected genes, the abundances of the mRNAs were analysed with qPCR.
In comparison, mmu-miR-503* could be detected, however, we could not confirm regulation.
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