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When kept in the dark for 24 hours, the genes' activity fluctuated randomly, confirming light's influence.
Control experiments were also performed by daylight confirming light sensitivity of SNO modifications.
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Theoretical and experimental results have confirmed light confinement as well as guided modes propagation by these new channel optical waveguides.
Because of the novelty of this observation, we confirmed light repression on a subset of transcripts by RT-qPCR.
The change of endogenous ASN1 transcript levels in response to various transient treatments of light and carbon (+L-C; +L+C; -L+C; -L-C) were compared in wild-type and cli mutants to confirm light and/or carbon misregulation of the endogenous ASN1 gene.
Whole-cell patch-clamp recordings in slice preparations confirmed light-evoked spiking at 5, 10, and 20 Hz, as well as during continuous light pulses of one second.
Loss of photocurrent could be largely prevented by rapidly closing the channels with green light, confirming that the light-gated channel was not degraded by the blue light used for stimulation (Fig. 2C).
Confirming the light microscopy examination, GR-ir was expressed more in the nucleus than in the perikaryon.
Confirming the light microscopic observations, we were also able to identify a small proportion of green and red double-positive cells within the tumors.
The classified failure mechanisms were confirmed by light microscopic images.
These velocities were confirmed using light obscuration, pressure measurement and particle image velocimetry methods.
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