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These results were confirmed using the same CML cell lines used previously (Figure S3 A C).

The expression of hTERT in transformed cells was also confirmed using the same methods (data not shown).

A further dilution series was then made from the undiluted RNA preparation (QIAamp method) to generate samples with Ct values of 15, 20, 25 and 30 and the expected level of FMDV RNA was confirmed using the same assay (data not shown).

The identity and expression of five fungal TDFs expressed in planta was confirmed using the same technique (Table 4).

These results were confirmed using the same analyses for gene lists derived from NF- κB-related GO identifiers (Table 2).

The purity of the isolated key intermediates and the final compounds was further confirmed using the same HPLC conditions.

Cells were subsequently transfected with 1 nM of either EFNB2 esiRNA or scrambled esiRNA for 72 h, after which EFNB2 silencing was confirmed using RT-PCR using the same primers outlined in Table 1.

Additionally, the other three lines showed the same (KY-10) or more NF-κB expression (TE-1 & TE-8) in the surviving lines, and this result was confirmed using immunocytochemistry with the same three lines (TE-1, TE-8, KY-10, data not shown).

Overall, results obtained by gene expression array could be confirmed using qPCR within the same donor (Additional file 1: Figure S3).

These findings were confirmed using same-level breast MRI images because CAD sometimes detected engorged axilla vessels as positive LN.

Digital PCR was performed to confirm genotypes using the same primer/probes.