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As it has been shown that the Ni- claMP complex acts as a superoxide mimic, verification of complex formation in the presence of EGF can be confirmed using a standard SOD activity assay.
Of the 1466 subjects who volunteered for this study, a total of 276 (18.8%) were identified as HBsAg + using a rapid screening test which was subsequently confirmed using a standard reference test (VIDAS, bioMérieux).
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Individual FAEEs were identified via their unique retention times and confirmed using a calibration standard composed of all FAEEs of interest (1,000 μg/ml; Cayman Chemicals, Ann Arbor, MI).
Furthermore, the samples that were negative by conventional methods and positive by real-time PCR could not be confirmed using a true gold standard because such a standard for respiratory viruses does not exist.
DNA markers were created by PCR amplification of virulence genes from Escherichia coli generating calculated-sized fragments (bp) of 881, 520, 337, 275 171 and relative sizes confirmed using a commercial DNA size standard (100 bp ladder, Promega).
Although false positive results are rare, positive reactivity in POCT should be confirmed by using a standard immunoassay such as an ELISA.
Positive results were confirmed by using a standard PCR specific for the ompA gene of Rickettsia spp. and the 16S rRNA and flaB genes of Borrelia spp. (8 ).
The cytotoxicity of curcumin on MeWo cells was confirmed by using a standard tetrazolium-based MTT assay (data not shown) to find the proper treatment concentration of curcumin.
For healthy controls additional exclusion criteria were any other neurological or psychiatric disease or a first or second degree relative with a diagnosis of MS. Episodes of MSON were identified through patient history and confirmed clinically using a standard care protocol.
A few targets were further confirmed using standard compounds.
Mauve colonies were plated to a blood agar plate and S. aureus was confirmed using standard microbiology procedures.
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