Sentence examples for confirmed transcript from inspiring English sources
Combined with results of several previous studies of inhibiting transcription and translation which have showed pollen germination and early tube growth strictly depend on protein synthesis but not transcription [ 14, 18- 20], our results confirmed transcript storage through pollen germination in rice.
Quantitative real-time PCR and western blot analysis for Esrp1 and Esrp2 confirmed transcript and protein ablation in vivo.
In addition, we confirmed transcript levels of 37 genes selected from both platforms by quantitative reverse transcriptase PCR (qRT-PCR, see Additional file 3).
RT-PCR analysis of CAFs confirmed transcript expression of mesenchymal markers vimentin and fibroblast-specific protein 1 (VIM, FSP1), smooth muscle markers (CNN1, MYH11), pro-tumorigenic chemokine CXCL12 (Orimo et al., 2005), reactive stroma protease fibroblast activated protein (FAP) and androgen receptor (supplementary material Fig. S2 and Table S1).
The isTSS located internal to BCAL0300 could not be confirmed, transcripts originating from this locus are probably truncated.
We tested whether ongoing transcription of these same superfamilies was also occurring in Ambystoma mexicanum using TBlastX against the A. mexicanum transcriptome (http://www.ambystoma.org/genome-resources/21-blast [date last accessed 26 Sep 2011]) and confirmed transcripts of all seven superfamilies.
PCR products were visualized by agarose gel electrophoresis to confirm transcript expression.
ESTScan v2 [ 39] was then used to confirm transcript coding regions and determine sequence orientation.
qRT-PCR was utilized to confirm transcript profiles obtained from RNAseq for selected genes.
CAP37 protein expression in the temporal and frontal lobes of patients with AD and normal controls was analyzed by Western blot to confirm transcript expression analysis.
In order to confirm transcript expression at protein level, we carried out a number of western blot experiments by utilizing affinity-purified antibodies selectively targeting Stim1, Stim2, Orai1, Orai3, TRPC1, and TRPC3/6/7 [ 10, 31, 32].
