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The synthesis of UNCD films using the uniquely focused plasma jet was confirmed to efficiently compensate for the sluggish kinetics and insufficient density of plasma species during the LPLT synthesis.
IgG isolation on the protein G column was confirmed to efficiently remove non-IgG materials.
Importantly, the MEK1/2 and PI3K inhibitors were confirmed to efficiently suppress serum-induced Erk1/2 and Akt phosphorylation, respectively.
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In all cases, the assembly of the prefabricated HAC vector was monitored by resolution of the individual species within the ligation reaction using Pulsed Field Gel Electrophoresis (PFGE) (Bio-rad, DR-III), or Field Inversion Gel Electrophoresis (FIGE) (Bio-rad) and confirmed to have proceeded efficiently as shown in Figure 2.
Active Freeze Drying has confirmed to be a promising tool to efficiently produce redispersible nanocrystal powders.
The ability of the nanoparticles to reduce the bulk powder cohesion has been confirmed to be dependent on how efficiently they can disrupt the direct contacts between the PS-DVB particles, resulting in a significant reduction of the interparticle adhesion.
The unexpected proliferation behavior of N-RAS61K-AR cells derived from senescent cell cultures was further confirmed by their capacity to efficiently form soft agar colonies.
This allows us to efficiently confirm the expression of 17 identified genes.
This has been biochemically confirmed, as Srs2 was shown to efficiently dismantle the Rad51-presynaptic filament (Krejci et al, 2003; Veaute et al, 2003).
As our in vitro characterisation had confirmed that we were able to efficiently control the expression of a functional wt TP53, we next aimed to determine the consequences of wt TP53 induction in vivo in an orthotopic context using the MiaPaCa-2-TREx-TP53 cell system.
ChIP-qPCR analysis performed in primary human keratinocytes and mouse epidermis confirmed that p63 efficiently bound both the identified regions and more strongly to p63BS2 in mouse epidermis (Fig. 3a), which contains at least two evolutionary conserved p63-binding sites.
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