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Moreover, a peptide YVQI in CRD2 was confirmed to bind to Src kinases, therefore regulating the phosphorylation and externalization of galectin-4 (Ideo et al., 2013).
MPEG-PLLD-Arg was confirmed to bind and deliver gene effectively, and its gene transfection efficiency was significantly higher than PEI-25k under its optimized condition.
Only a decade later was it confirmed to bind to DNA and turn on the expression of other genes aimed at healing cell damage.
De Groot et al. applied a bioinformatics approach for predicting HLA-B*07 restricted WNV CD8+ T cell epitopes [8]: Out of 16 predicted epitopes, 12 were confirmed to bind HLA-B*07 in vitro, but the peptides' ability to induce T-cell responses was not tested.
Importantly, rFRβ was confirmed to bind to its ligand, folate.
One aptamer containing the found motif has also been experimentally confirmed to bind to the target.
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In summary, of 304 'apparently relevant' in vivo AdpA-binding sites, 284 sites (93%) were confirmed to be bound by AdpA in vitro.
Interestingly, two of them, ompT and yagU have been confirmed to be bound by Lrp in vivo using ChIP-qPCR [4].
Of these regions, 17 were confirmed to be bound by STAT6.
Two compounds, benzbromarone and diclazuril, were confirmed to be bound to PA and decreased the activity of the viral RNA polymerase.
ChIP-PCR experiments showed that these predictions were for the most part accurate – seven out of the ten positive targets microarray analysis were confirmed to be bound to RNA polymerase II.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com