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Site-directed mutagenesis studies have confirmed the function of the catalytic amino acids (Tyr154, Lys 158, Ser124 in both isoenzymes), substrate/inhibitor isoform-specific residues (His156 and Arg195 in 3βHSD1) and cofactor binding residues (Asp36 provides NAD+ specificity in both isoenzymes).
Many studies have confirmed the function of sialyl Lea and sialyl Lex in animal models of these diseases and the inhibition of binding of sialyl Lea and sialyl Lex to the selectins is a validated drug target in the pharmaceutical industry.
Using RNAi knockdown mosquitoes we confirmed the function of three different AQPs in the MTs of Ae. aegypti.
It was imperative that we confirmed the function of AgOXT1 before pursuing knockdown approaches.
We next confirmed the function of IL-32 with our retroviral construct, MIG-hIL-32β.
We confirmed the function of the HPA using a separate dataset from 22 admissions of type 1 diabetic subjects.
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Biochemical analyses of candidate genes have confirmed the functions of many genes involved in the assembly of grape pigments, flavors, and aromas, as well as a number of regulatory genes.
Our results confirmed the functions of this protein.
Knockout experiments have confirmed the functions of both a siderophore biosynthesis protein and receptor from the acinetobactin gene cluster [ 24].
This confirms the function of reelin in specific targeting at vLGN and IGL but not dLGN.
To confirm the function of GA as adult phase promoter, I examined the phenotypes of GA deficient mutant, d18-dy.
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