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Site-directed mutagenesis studies have confirmed the function of the catalytic amino acids (Tyr154, Lys 158, Ser124 in both isoenzymes), substrate/inhibitor isoform-specific residues (His156 and Arg195 in 3βHSD1) and cofactor binding residues (Asp36 provides NAD+ specificity in both isoenzymes).
Many studies have confirmed the function of sialyl Lea and sialyl Lex in animal models of these diseases and the inhibition of binding of sialyl Lea and sialyl Lex to the selectins is a validated drug target in the pharmaceutical industry.
Using RNAi knockdown mosquitoes we confirmed the function of three different AQPs in the MTs of Ae. aegypti.
It was imperative that we confirmed the function of AgOXT1 before pursuing knockdown approaches.
We next confirmed the function of IL-32 with our retroviral construct, MIG-hIL-32β.
We confirmed the function of the HPA using a separate dataset from 22 admissions of type 1 diabetic subjects.
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Our results confirmed the functions of this protein.
Once they are confirmed, the function-describing sentences are instantly submitted to our database and then can be accessed by other users.
Knockout experiments have confirmed the functions of both a siderophore biosynthesis protein and receptor from the acinetobactin gene cluster [ 24].
This confirms the function of reelin in specific targeting at vLGN and IGL but not dLGN.
It has also been efficiently used in confirming the function of miRNAs such as miR399 in response to Pi stress.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com