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Two weeks after differentiation, we confirmed the expression levels of Sox17, α-smooth muscle actin (SMA) and βIII tubulin by immunostaining (Figure S4).
We confirmed the expression levels of OsPORA mRNA in mature leaves of the transgenic lines.
Western blot further confirmed the expression levels observed in the immunostaining and the mRNA levels detected by the RT-PCR (Figure 3C).
Additionally, we confirmed the expression levels of their corresponding targets by qRT-PCR (Fig. 5c).
We confirmed the expression levels using both Western blot and qRT-PCR.
Real time-PCR experiments confirmed the expression levels of the selected CaRGAs.
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We confirmed the expression level of miR-210 using quantitative real-time PCR analysis.
RT-qPCR experiments of randomly selected genes confirmed the expression level of genes of interest using FPKM (Additional files 6, 7 and 8).
The gene specific primers were used to confirm the expression levels by qRT-PCR.
qRT-PCR was used to confirm the expression levels of mRNAs.
Using this criteria we were able to confirm the expression levels of 7/8 up-regulated miRNAs and 1/6 down-regulated miRNAs, shown in Table 1.
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