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We also confirmed the down regulation of CXCL12 by miR-886-3p miR-886-3p miR-886-3pines (U87MG, A375, T47D and MCF7, data not shown).

In support of this, qRT-PCR confirmed the down regulation of two VSMC-differentiation markers, smooth muscle α-actin and smooth muscle myosin heavy chain, in the aorta of adult miR-143(145) KO mouse compared to WT (Fig. 6b).

Further immunoblotting confirmed the down-regulations of MT-ATP8, COX2, NDUFA6, and SDHB, four selected proteins among these 20 proteins.

Immunofluorescence analyses confirmed the down-regulated expression of some of these genes, in particular of the cartilage oligomeric matrix protein and osteopontin, encoded by COMP and SPP1, respectively, and showed the predominant reduction and disassembly of the heparan sulfate specific GAGs, as well as of the PG perlecan and type III and V collagens.

Quantification analysis through qPCR further confirmed the down-regulated changes of nkx2.2a and ptc1 expression (Figure 2T).

Western blot analysis confirmed the down-regulation of STAT5b expression in MHCC97L-PRNK cells compared to that in MHCC97L-vector cells (Fig. 4D).

RT-PCR validation on individual pancreatic RNA samples confirmed the down-regulation of Nnat observed in Hnf6 Tg animals on the microarray (Figure 3).

Differential Display results confirmed the down-regulation of c-myc and showed down-regulation of p53.

Our results confirmed the down-regulation of miR-223 in IGHV UM CLLs.

qRT-PCR also confirmed the down-regulation of Pomc, Lhb, Fshb in rat adenomas (Fig.  1b).

The results of ELISA also confirmed the down-regulation of CCL2 expression in the treated cells at the protein level.