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We then confirmed that p110α indeed specifically binds to BMPRII by precipitation of endogenous p110α which co-immunoprecipitated BMPRII in a BMP2-dependent manner.

This confirms that CTSB specifically cleaves the GFLG chain rather than PEG chain.

However, we could not confirm that citrinin specifically affects mitochondria.

This would confirm that HCAb2 specifically localized to MDA-MB-231 xenograft tumor cells.

To confirm that LPA specifically activates the p42/44 MAPK cascade, different inhibitors of the MAPK pathway were used.

Furthermore, we were able to confirm that omalizumab specifically blocked the binding between IgE and FcεRI (Figs 2b, 3b).

We were able to confirm that SptP specifically dephosphorylates host VCP during cell infection, and this did not occur when the PTPase active site was disabled.

To confirm that fibrinogen specifically bound ICAM-1, we pre-treated isolated cardiomyocytes with either with an ICAM-1-blocking antibody or non-specific IgG.

To confirm that BHA specifically blocked the differentiation of monocytes to M2, but not M1 macrophages, we examined the expression of M1 and M2 macrophage-specific markers.

To confirm that PODOC1 specifically binds to podocalyxin, Western blot analysis was performed on shCTRL and shPODXL MDA-MB-231 cell lysates.

Real-time quantitative PCR was used for several genomic regions that included known methylated or unmethylated promoters to confirm that MeDIP specifically enriched methylated DNA and efficiently removed unmethylated DNA (Additional file 1a).