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In this study, 7 isolates were identified as RIF-resistant by the Xpert assays; 6 of these isolates had confirmed resistance according to culture-based DST.
Washing fastness tests confirmed resistance of the samples to at least 50 washings.
The results confirm strobilurin resistance from 2003 in the Microdochium populations of wheat and also confirmed resistance in barley for the first time.
IR66 with bph4 gene for resistance was released in 1987 and IR68, IR70, IR72 and IR74 with Bph 3 gene were released in 1988 with confirmed resistance to biotype 3 (Table 2).
We confirmed resistance to Tzb by performing MTT-based cell viability assays.
In swine, Roepstorff et al. [ 4] confirmed resistance to pyrantel citrate in Oesophagostomum spp. in Denmark.
Similar(48)
Once transformed, these clones were evaluated to confirm resistance phenotypes and assess cross-resistance to other aminoglycosides.
Ten clones were taken randomly from each selective plate to identify genomic regions contained within plasmid DNA and confirm resistance phenotypes.
MTT and neutral red uptake assay were performed to confirm resistance to paclitaxel.
A standard agar-proportion method with Middlebrook 7H10 media was used to confirm resistance (16 – 16 ).
64 The application of similar measures in future studies will robustly confirm resistance and allow us to detect non-concordance.
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