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The nurses confirmed recruitment of each patient by telephone to the lead investigator (JA), who accounted for all envelopes.
The increase of the immature PMN count and immature/ total PMN ratio confirmed recruitment of immature neutrophils from the bone marrow into the circulation.
Injection of OVA into the skin of an OVA-immunized mouse confirmed recruitment of neutrophils at a peripheral site following immune complex formation (Fig. 4m, n).
Chromatin fractionation followed by immunoblotting confirmed recruitment of RPA to chromatin in otherwise unperturbed Pol κ-depleted cells by using two independent siRNAs at levels comparable to what observed after UV treatment, which served here as a positive control for replicative stress (lane UV).
Indeed, chromatin immunoprecipitation (ChIP) assay confirmed recruitment and direct binding of HIF-1α to the STI-1 promoter in PCCs subjected to 4 h-hypoxia, but not in PCCs subjected to 4 h-normoxia or PCCs treated with LV-HIF-1α-shRNA with 4 h-hypoxia (Fig 3E).
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Participants were approached in person by the Principal Investigator (MJ) at staff meetings and research staff then sent follow up emails and made phone calls to confirm recruitment and schedule visits.
This discrepancy confirmed the recruitment of macrophage during M. bovis infection, which conforms to the result of previous report.
Chromatin immunoprecipitation (ChIP) assay confirmed the recruitment of transcription factors phospho ATF-2, Sp1, Sp3, PPARγ, chromatin modifier histone deacetylase 1 (HDand) and the acetylated form of histone H3 but not cRel, C/EBP-α and phospho c-Jun during resistin gene transcription.
Our data are also consistent with the notion that Myc interacts with multiple HATs and recruits these enzymes to its binding sites in chromatin, thereby enhancing histone acetylation: we confirmed the recruitment of Tip60 [26] and demonstrated that of the related enzyme HBO1, both of which preferentially acetylate histone H4 [41].
We confirmed Akt recruitment to the membrane after insulin activation by quantitative immunofluorescence.
The experiment confirmed the recruitment of NKX3.1 to NBS1 and NBS4 regions.
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