Invoices for photocopying and other production expenses were filed by research administrators and used as the bases for cost estimates and to confirm quantities.
Relevant data on the port is shown in Table 2. Before the open date, ten crude oil cargoes are identified, of which seven cargoes have confirmed (offered) quantity and freight and three unconfirmed cargoes (not yet offered).
Soil sample analyses confirmed significant quantities of lead in the sediments.
PCR products were separated on a 2% agarose gel to confirm size, quantity and purity of each product, and then purified using NucleoSpin Kit (Macherey-Nagel), according to manufacturer's instructions.
Following baseline assessments, a physiotherapist will collect the appropriate envelope after confirming sputum quantity.
Inserts were amplified for 36 cycles (95°C for 30 sec, 54°C for 30 sec, 72°C for 2 min), with an initial denaturation at 95°C for 2 min and final extension at 72°C for 6 min. Two (2) μl of each reaction were separated on 1.5% agarose gels to confirm amplification quantity and quality.
To confirm the quantity of viral copies in the diluted HIV-1 infected ACH-2 cell suspension, at a later date, the DNA was extracted using the Qiagen DNA extraction kit, and HIV copy number was quantified using HIV-1 pol real-time PCR [ 14, 15].
Duplicate samples of proteins (50 to 100 μg) were resolved on 11% (w/v) polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes by semi-dry blotting (Owl Scientific), or stained with Coomassie blue to confirm protein quantity and assess protein integrity.
In vivo study in a rat calvarium model confirmed a higher quantity of new bone formation in the fiber linked with fusion protein, and a further increase was noticed when the MSCs were tissue-engineered with the fusion protein-linked fiber.
More hydrolysis of the hemicellulose in pretreated water hyacinth and rice husk was also confirmed by minute quantity of xylose in the enzymatic hydrolyzates).
The elemental surface composition analysis carried out by EDX (Fig. 1b) confirms stoichiometric quantities of Ti and O in for TiO2.
