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The UK's Food Standards Agency said Mars had confirmed products which had been recalled in the Netherlands and Germany had been distributed in the UK.
Histopathologic examination confirmed products of conception in 537 (96.2%) patients, no products of conception in 17 (3%) patients, molar pregnancy in 2 (0.4%) patients, and decidual tissues without chorionic villi (Arias-Stella reaction) in 2 (0.4%) patients.
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The presence of a single melting peak followed by analysis on 1.5% agarose gel confirmed product specificity.
We confirmed product purity by DNA melting curve analysis.
We confirmed product purity by melting curve analysis.
Melting curves were performed to document single product formation and agarose electrophoresis confirmed product size.
Analyses on gel electrophoresis (Scheme 1) and HPLC confirmed product purities.
In addition, the specific melting point (MP) of the amplified product carried out within the LightCycler standard PCR protocol confirmed product identity [ 49].
Forty cycles of PCR were used in each Q RT-PCR reaction with a dissociation step to confirm products.
For each experiment performed amplification reactions were resolved on agarose gels to confirm products of the predicted size for each gene were amplified (example shown in Figure 1).
To confirm product specificity, a melting curve analysis was performed after each amplification.
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