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The presence of a single melting peak followed by analysis on 1.5% agarose gel confirmed product specificity.
We confirmed product purity by DNA melting curve analysis.
We confirmed product purity by melting curve analysis.
Analyses on gel electrophoresis (Scheme 1) and HPLC confirmed product purities.
Melting curves were performed to document single product formation and agarose electrophoresis confirmed product size.
In addition, the specific melting point (MP) of the amplified product carried out within the LightCycler standard PCR protocol confirmed product identity [ 49].
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The UK's Food Standards Agency said Mars had confirmed products which had been recalled in the Netherlands and Germany had been distributed in the UK.
To confirm product specificity, a melting curve analysis was performed after each amplification.
After the 50 cycles, a melting curve analysis (55°C to 95°C) was performed to confirm product specificity.
Gene expression was analyzed by Gene Expression Macro (Bio-Rad) and PCR products were also analyzed by 2% agarose gel electrophoresis to confirm product size and specificity.
Gel images were captured digitally to confirm product size and the absence of non-specific products.
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