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Quantitative bisulphite validation studies confirmed our ability to discriminate differences in methylation in these samples, and the biological coherence of results in terms of functional pathway relatedness is suggestive of underlying changes in epigenetic regulation as a response to IUGR.
Consequently, we confirmed our ability to silence RAD54B and SOD1 and performed dual siRNA experiments in RAD54B-proficient parental cells.
We confirmed our ability to detect co-labeling by examining the histaminergic tuberomamillary nucleus (TMN) and dopaminergic A13 cell group, both of which express GADs (Lin et al., 1990; Esclapez et al., 1993).
In addition, a supervised motif enrichment test on putative enhancer regions using Clover [ 27] confirmed our ability to identify known TFs centrally involved in ES cell transcriptional regulatory networks.
We confirmed our ability to detect reduced FST immobility in an independent cohort of rats using an established subchronic administration of the antidepressant imipramine, which significantly reduced immobility times (104 ± 3.2 s, mean ± SEM) compared with rats injected with vehicle (185.7 ± 19.8 s; n = 3 rats/treatment group; Student's t test p = 0.014).
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We have a proven track record in raising capital to fund strategic initiatives and have received a Highly Confident Letter from our financial advisor, Barclays, confirming our ability to raise the required financing.
None of the 4 Rag2-deficient control mice whose fat pads (n = 8) were cleared but not injected with mammary epithelial cells contained ductal out-growths, confirming our ability to successfully clear the mammary fat pad (Fig. 3D).
The 300 least-covered fragments (Table S1) included HML and HMR, the silenced mating cassettes, confirming our ability to detect areas that do not shear well due to silencing.
In both regions, ChR2+ neurons were immunopositive for ChAT, confirming our ability to selectively activate endogenous Chat+ inputs to cortex.
All positive cultures were confirmed to be M. pachydermatis by PCR, which confirms our ability to identify the species properly by cytology.
They also confirm our ability to categorize infections correctly, because it would not be possible to create a workable ranking otherwise.
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