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Specificity of immunolabeling was tested and confirmed on samples from Kv4.2−/− mice (Fig. 1d).
Specificity of the antibodies in immunolabeling experiments was tested and confirmed on samples from Kv4.2−/− mice.
Microscopic examinations were made with Ziehl-Neelsen coloration and confirmed on samples of sputum or bronchial aspirate using trough techniques of gene amplification (PCR), cultures on solid media (Lowenstein-Jensen) and liquid, and radiometric technique BACTEC MGIT 960.
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Discordant results of rapid tests were confirmed on serum samples by ELISA (Vironostika, Biomirieux, Durham NC, USA).
Differential expression of RECK was confirmed on independent samples from the same cases (Figs. 2 and 3).
Tumour load was confirmed on BM samples.
The prognostic performance of the PCR-GGI was confirmed on FFPE samples (HRunivar. = 1.89; [95CI:1.01-3.54], p = 0.05).
DNA methylation levels were further confirmed on independent samples by pyrosequencing of the promoter sequences.
These mutations were confirmed on independent samples by direct Sanger sequencing.
A diagnosis of EBLV-2 infection was confirmed on brain samples with a fluorescent-antibody test, the mouse inoculation test, and a rapid TaqMan assay (8 ).
The specificity of immunoreaction was tested and confirmed on tissue samples from Kv4.2−/− mice (deletion of the KCND2 locus by homologous recombination; Guo et al. 2005).
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