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Every connection found by Pathway Studio was confirmed manually and incorrectly associated interactions were excluded.
All genotypes were confirmed manually and passed standard quality control checks (see supplementary methods, available online only).
The quality of each SNP was confirmed manually, and a few of the SNPs of low quality and a minor allele frequency < 5 % were removed from the dataset.
Sequence reads were confirmed manually and complete mtDNA sequences were assembled using the SeqMan module of the Lasergene 8.0 software (DNASTAR, Madison, WI, USA).
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Alignments and haplotypes were identified and confirmed manually in Bioedit v. 5.0.6 [45.0.6
Sequence data was edited and aligned using DNASTAR DNASTARR, Inc ., then inspected and confirmed manually.
tRNA genes were identified by their proposed cloverleaf secondary structure and anti-codon sequences using tRNAscan-SE1.21[ 79] and ARWEN [ 80] with relaxed settings, and confirmed manually.
In addition, all results (emitter pair correlations) had to be checked and confirmed manually.
Arrhythmias were identified using an automated algorithm and confirmed manually by trained operators.
The result (as well as many other variants across the 24 samples not described herein) was checked and confirmed manually.
We matched spots from the different gels using the automatic matching function in the Biological Variance Analysis DeCyder module after prior landmark settings and confirmed manually by comparing each spot against the master gel.
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CEO of Professional Science Editing for Scientists @ prosciediting.com