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Quantitative PCR analysis confirmed large differences in transcript abundance between P18 and U18, with much smaller changes between P12 and U12 and P24 and U24.
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Further analysis based on amino acid composition profiles confirmed large differences between various species.
For NF, ion flux simulations confirm that large differences in the permeances of mono and divalent ions, e.g. Mg2+ and Cl− or Na+ and SO2−4, can lead to negative rejections or enhanced rejections of trace monovalent ions, depending on the ion charge.
We confirm expected large differences in the samples' response to thermal treatment.
Boxplots of beta methylation values of the 67 CpGs confirmed the relatively large differences in methylation between HOTAIR expressors and non-expressors (Additional file 11).
(A″ ) Stable amount of puncta and non-puncta in the pre-pupal wing confirms a large difference in stability between regions.
Although these studies considered much shorter time-scales (up to 24 hours using cell culture) than in the present study, they do confirm that there are large differences in gene expression with time.
The average judgments for each of the different conditions are presented in Figure 4 A, and these findings confirm that there were large differences in apparent depth between the 3D shapes and the 2D controls for the texture stimuli.
To confirm that there are no large differences in protein composition of thylakoids and BBY particles (except PsbS protein) in wild-type and PsbS-KO plants, we have conducted SDS-PAGE with Coomassie staining (Additional file 1: Figure S11), and the result showed that there are no large differences in the protein compositions between wild-type and PsbS-KO plants.
It is possible that the tested range of difference in RS content in that study was not sufficient to observe a change in the GI, which is confirmed by the fact that only large differences in amylose content (leading to high RS content after cooking and cooling) lead to relatively large effects on the GI.
For these shorter chain-length PHA polymers the identity was confirmed by 1H-NMR due to the large differences in production between strains (Additional file 1: Figure S2).
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