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We identified necessary network module for each mode (Fig. 2D) and confirmed its function for the stripe formation (Fig. 3A C and Result S1).
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Purification and reconstitution of E. coli BioH activity in vitro confirmed its function.
Future studies in primary T cells and macrophages are warranted to confirm its function.
The sgll knockdown further confirms its function as the Drosophila PNPO.
pQWD2 was transformed into EPEC espD mutant strain UMD870 to confirm its function by complementation (6).
Studies with CTLA-4−/− mice confirmed its inhibitory function in vivo.
Moreover, the expression of TMC1 gene confirmed its important function in echolocating bats.
N-acetylornithine was accumulated in ∆ argRJ, which confirmed its anticipated function as ornithine acetyl transferase (OAT).
We used these characteristics to predict the metabolic involvement for a Transcriptional Regulator (TR) of unknown function, identified and confirmed its biological activity.
In conclusion, our systematic analysis of BH4 function in C. elegans confirms its essential role as a cofactor for TH and TPH in neurotransmitter synthesis in neurons.
Tissue-specific knockout of NELF-B confirms its cell-autonomous function in cardiomyocytes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com