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Forward and reverse primers (Table 1) for the aforementioned genes were designed based on sequences available in GenBank (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi) using the MIT Primer 3 designer software (http://wi.mit.edu/cgi-bin/primer3/primer3_www.cgi), and were confirmed for specificity using the basic local alignment search tool (www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
All primers were confirmed for specificity via cloning and sequencing prior to use (data not shown).
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The generated antibodies were confirmed for their specificity by binding to type-specific HDAgs expressed in DNA-transfected Huh-7 hepatoma cells.
All primers were confirmed for gene specificity using BLAST Basic Local Alignment Search Tooll) (Genbank database sequences).
In a common tier based approach, samples identified as ADA screen positive are confirmed for the binding specificity of the antibodies to the drug molecule via a confirmatory assay.
The results obtained by the Affymetrix GeneChip® arrays (Table 1) were confirmed for OxLDL and specificity of the change for HIHG conditions was demonstrated.
Primer specificity was confirmed for reference strains of Clostridium botulinum types B and E for strains representing bacterial species common in food, and for the DNA mixtures of C. botulinum types B and E in the presence of background DNA isolated from cold smoked salmon and ham.
Peptide specificity was confirmed for Abs induced by the 15/3/6- and the 43.12p3-KLH conjugate, as they bound to the corresponding peptide-BSA conjugates (Figure 4).
Template specificity was confirmed for all primer pairs by semiquantitative PCR with cDNA and gDNA templates.
The highest specificity was confirmed for the two newest models (GASH and ABIC), but CPT, MELD, and MELD-Na were better regarding a higher sensitivity.
Primer were designed (Table 9), and their specificity was confirmed for each gene by a unique pick of the fusion curve after real-time RT-PCR amplification.
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