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Antibodies to detect total levels of STAT3 and GAPDH confirmed equivalent loading in each case.

As an additional control, we further confirmed equivalent protein loading by stripping the same blots and re-blotting them with antibody to actin (Figure 2E, lower panel).

Ponceau staining confirmed equivalent loading of each sample and membranes were then blocked in 5% milk in TBST buffer, and probed with the primary antibody.

Western blotting of the kinase proteins pulled down by the GST-c-Jun beads confirmed equivalent (saturated) amounts of wildtype and optimized JNK1 (Fig. 4B, upper blot).

Western blotting confirmed equivalent induction of RTA protein in the vector- and K13-expressing cells, thereby arguing against the possibility that the increased survival of K13-expressing cells was due to their inability to induce RTA expression upon doxycycline treatment (Figure 5B).

Western blotting confirmed equivalent induction of RTA upon doxycycline treatment in the empty vector and K13-ERTAM cells in the absence or presence of 4OHT (Figure 2C), thereby arguing against the possibility that the lack of expression of lytic proteins in K13-ERTAM cells is due to the loss of doxycycline-induced RTA induction.

Western blot confirmed equivalent expression of both wild-type and mutant HIWI in Miwi siR-treated cells.

Several studies have confirmed equivalent effectiveness, mortality and fluid balance status compared with hemodialysis, at least for the first 4 5 years.

36 The B2303 study was a randomized trial of the AL dispersible formulation versus AL tablets administered crushed: patients receiving the dispersible tablet were only included in the safety analyses (data from the B2303 study population confirmed equivalent efficacy and safety of the dispersible formulation and the crushed AL tablets).

In 2009, there were 65 invasive MenY cases (46 culture-confirmed and 19 PCR-confirmed), equivalent to 1.2 cases per million population (Table 2).

All mice were imaged optically to confirm equivalent infection levels prior to [18F]FDG-6-P or [18F]FDG administration.