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The identification of C. fayeri by SSU rRNA was confirmed by the sequence of the actin gene (8 ), showing 99.8% similarity to C. fayeri (GenBank accession no. AF112570).
For this reason, the qPCR system provides merely an indirect proof of the presence of GMO in a food/feed matrix since it could only be confirmed by the sequence of their transgene flanking regions.
Clone D from KYM-1 cells showed both fragment lengths corresponded with an un-edited fragment length, and this was confirmed by the sequence data, which showed a 1 bp alteration in both alleles.
Clone D from KYM-1 cells showed both fragment lengths corresponded with an un-edited fragment length, and this was confirmed by the sequence data, which showed a single nucleotide alteration in both alleles; this mutation disrupts the FauI site, but retains the reading frame, suggesting that EZH2 remains expressed (and likely functional) in these clones.
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The presence of the correct mutation was confirmed by sequencing (The Sequencing Service, University of Dundee).
The coding sequences of all plasmids constructed in this work were confirmed by sequencing (The Sequencing Service, University of Dundee).
The genotypes were confirmed by the DNA sequencing analysis.
These ORFs were confirmed by sequencing, and the sequences were submitted to GenBank (Table 1).
Genotyping was confirmed by using the sequences obtained above.
Differences with respect to the reference sequence were confirmed by sequencing the complementary strand.
The specificity of the insert was confirmed by sequencing with the M13 sequencing primers.
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