Prediction data were preliminary confirmed by expression analysis of the identified miRNAs in androgen-dependent (LNCaP) and independent (PC3) prostate carcinoma cell lines and in normal prostatic epithelial cells (PrEC).
This hypothesis was confirmed by expression analysis.
Fidelity of the nuclear and cytoplasmic fractions was confirmed by expression of nucleolin/C23 and β-actin.
This transition observed by hepatic gene expression analysis is confirmed by expression of inflammatory proteins in plasma and the late increase in hepatic triglyceride content.
Ten intracellular esterases, five with activity confirmed by expression in E. coli, and five putative esterases were also found in the P. freudenreichii genome [36].
After day 4 and day 8, rES cell status was confirmed by expression of mRNAs for ES cell specific transcription factors Nanog, Oct3/4 and Sox2.
The importance of the missing tail region from the P100 R4227X mutant was confirmed by expression of an artificial PC1 product, AESW, containing only the final transmembrane domain and the C-terminal tail in Xenopus oocytes.
The absence of TLR4 expression in differentiated KCs, which was confirmed by expression microarray (see below), is consistent with work by others showing that TLR4 was only found in HaCat cells, but not in primary human KCs [16], [48].
