Sentence examples for confirmed by adding a from inspiring English sources

The absence of catalase activity in "military" isolates was confirmed by adding a solution of 3% H2O2 to smears of various strains.

The significance of the methylation status upon MGMT expression in vitro was confirmed by adding a demethylating agent to SNU-620, which does not express MGMT mRNA.

Furthermore, the presence of the individual flavonoids and isoflavones in the urine matrix was confirmed by adding a mixed standard solution to the urine samples.

Specificity was confirmed by adding a combination of the NPY receptor antagonists: 10 µM BIBO 3304 for Y1; 10 µM BIIE 0246 for Y2; 100 µM L-152,804 for Y5.

The data were confirmed by adding back an excess of purified CD4+CD25+ T cells to autologous T cells.

Persistent expression was confirmed by adding luciferin substrate and imaging the flask on an IVIS 200 system (Xenogen, Alameda, CA), and by western blot analysis prior to use.

The specific interaction was confirmed by adding the p53 antibody into the binding reaction, resulting in a supershift band (antibody/p53/RASSF1A) (Fig. 3A, lane 2).

This qualitative assessment was confirmed by adding the shuffled baseline to the ANOVA for condition, which produced a significant effect (F (2, 122) = 73.727, p < 0.001, ηp2 = 0.551).

The tight regulation of HSF1 expression levels by SIRT1 was further confirmed by adding sirtuin inhibitors, sirtinol or nicotinamide (NAM), or co-transfection with a dominant-negative (DN) SIRT1 H355A defective in catalytic function (Fig. 5E and Supplementary Material, Fig. S4A).

That TGF-β3 activity induced in the mouse serum is a vital ingredient for this effect was confirmed by adding TGF-β3 neutralizer to the medium containing serum from irradiated mice before transfer to the reporter cells.

The identity of the transcriptional element was confirmed by adding the antibody to the p50 subunit of NF- κB to demonstrate a supershifted complex (lane 6).