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VCL gives rise to one confirmed alternative splice variant, metavinculin, which is preferentially expressed in muscle tissue [2], [3].
To further verify the precise quantitative patterns of exon inclusion of the genes where the end-point quantification was not conclusive, we selected five of the already confirmed alternative cassette exons for analysis using quantitative real-time PCR.
Unsuccessful treatment outcome includes treatment failure (excluding three patients with confirmed alternative diagnosis), default, death, or lost to follow-up at final evaluation.
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The central question, he argues, is whether we should believe that there are well confirmed alternatives to our best scientific theories that are presently unconceived by us.
Observations of non-ravens, however, unlike those of black ravens, confirm alternative hypotheses, like "All ravens are blue" and "All ravens are green".
Table S1 shows the primers used to confirm alternative splicing of the MTT5 intron.
MTC performed RT-PCR to confirm alternative splicing.
In some cases cDNAs confirm alternative use of the two boundaries in JEC21.
A differential diagnosis should be formulated and additional investigations should be performed to rule out or confirm alternative diagnoses.
These findings confirm alternative genetic mechanisms, beyond allelic loss, are responsible for loss of PTEN protein expression.
In order to confirm alternative splice sites in the bovine SILV gene, we developed splice variant specific RT-PCR tests for an exemplary subset of the detected splice variants (Table 3, Figure 4).
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