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Post CO2 sequestration NMR and MICP analysis also confirmed altered pore character in the storage samples.
Nevertheless, expression patterns obtained via qRT-PCR confirmed altered expression of these genes under various abiotic stress conditions.
Real-time RT-PCR analysis confirmed altered expression of p53 targets such as p21CIP1 and Pltp, and down-regulation of p21CIP1 downstream genes such as Ube2c, Ccdc99 and Tubb3 in FHL2−/− cells (Fig. 4C).
Additionally, these assays confirmed altered B lymphopoiesis in the collagen X Tg and KO mice, with dramatic reduction in pre-B cell colony outgrowth from the perinatal lethal subset, all of which supports the B220+, CD138+, IgD+, and IgM+ flow cytometry from marrow derived lymphocytes (Figs. 2B,C, 5D) [7], [7].
Further, flow cytometry confirmed altered B lymphopoiesis, which revealed diminished B220+, CD138+, IgM+, and IgD+ lymphocytes from marrows of all outbred and congenic collagen X Tg and KO mice throughout life, with most dramatic reductions in the perinatal lethal mice (Fig. 2B and [5]).
Quantitative real time reverse transcription-polymerase chain reaction confirmed altered expression of several genes.
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Western blotting was used to further confirm altered SERPINB2 expression in the toxicant-exposed stem cells (Fig. 3c).
Figure 4 qRT-PCR confirms altered expression of few yield-related genes under abiotic stress conditions.
The pre-treatment measure confirms altered cortical information processing in migraine patients.
To some extent this has already been investigated but without confirming altered amygdala activation in alexithymia [17], [45], [46].
2-D immunoblotting with highly specific primary antibodies was employed to confirm altered expression levels of distinct quadriceps muscle proteins.
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