The American account said that surveillance confirmed "activity consistent with the reports, and supporting aircraft engaged the time-sensitive target".
Moreover, recent in vitro studies also confirmed activity against all Gram-positive isolates, which were inhibited by ≤1 μg/mL tedizolid in samples of patients with HAP.
We identified two hits with validated structures and confirmed activity in the primary assay (IC50 values = 322 nM and 7.7 μM) and two orthogonal MEKK2 biochemical assays.
At the chosen cutoff level, there was only little agreement between predicted and confirmed activity.
Of the 50 compounds retested in the ARE- bla and ARE- luc assays, 47 (94%) and 45 (90%), respectively, had confirmed activity between the primary screening and follow-up studies.
91 resupplied compounds confirmed activity with 30 hits exhibiting IC50 values ≤10 μM; among them was SKI-356313 with a confirmed IC50 value of 2.54 μM.
Further screening (at the Infectious Disease Research Institute, Seattle, WA, USA) confirmed activity against Mtb (MIC of 10 11 μM in 7H9-Tw-OADC media).
Greater than 80% of the HTS fractions confirmed activity at retest, which was performed in a 5-point dose response against both 3D7 and Dd2 parasite strains.
Preclinical and early phase clinical studies confirmed activity of sunitinib in urothelial cancer and showed that it could be combined with GC cytotoxic chemotherapy [5 7].
