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Patient volume and confirmatory algorithms should also be considered [1].
Details are in Tables 3 and 4.> -wrap-foot>> *DiscoRDTnt resultsults are reported separately because in the confirmatory algorithms (eg KHB-Unigold-OIC), a serological confirmation test is only indicated if both test 1 and test 2 are positive.
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In addition, users should develop an appropriate testing and confirmatory algorithm to ensure mutant detection.
Further guidance on follow-up of an abnormal NBS test for PHE can be found in the ACMG ACT sheets for newborns along with confirmatory testing algorithms (www.acmg.net).net
There was complete concordance between the rapid test algorithm and confirmatory tests, resulting in excellent specificity and negative predictive value (100.0%, CI 97.8 100.0% for both indicators), as well as sensitivity and positive predictive value (100%, CI 59.0 100.0% for both indicators) albeit with a wide confidence interval reflecting the small number of true positives in the sample.
The exomes of six BRC-responsive prolactinomas and six BRC-resistant prolactinomas were analyzed by whole-exome sequencing which yielded excellent target region coverage with approximately 71% of the exome covered to a depth of at least ten-fold between the somatic variant calling algorithm and confirmatory sequencing.
Furthermore, computational algorithms and multiple confirmatory assay formats combined with high-throughput screening methodologies greatly contribute to the identification and characterization of novel potent drug targets [ 67].
21 Therefore, before such tests are introduced in public health settings, it is necessary to envision a clear action plan this action plan includes a seamless integration of downstream confirmatory tests as per standard algorithms; integrating results from preliminary multiplexed POC devices will be essential to ensure rapid clinical action on the initial multiplexed screening result.
Additional file 4 shows a comparison of the results from the rapid test algorithm and the confirmatory testing.
The addition of the OIC confirmatory test to the KHB/STATPAK® algorithm eliminated the false positive result.
Although many endemic countries have standard definitions for clinical disease, definitions, algorithms, and protocols for confirmatory, laboratory diagnosis may differ within and between countries.
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