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Information on referral health care facility and date of laboratory test confirmation was collected from the NTBRL data.
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Samples for laboratory confirmation were collected and processed as described below.
Specimens for confirmation were collected at the age of 15.8±3.8 days (range 7 21 days).
Culture supernatants for ELISA and cells for Western blot confirmations were collected after 24 h of exposure.
Data on clinical signs and symptoms were collected for all subjects, and an additional NTS was collected for later confirmation of influenza infection by viral culture.
During follow-up, when a laboratory technician was not available to read a thick blood smear for malaria parasites, the Core™ Malaria Pf test (CORE Diagnostics, Birmingham, UK) was used to guide treatment and, a thick blood smear was collected for subsequent confirmation of the diagnosis.
After confirmation of an antigenic response, serum was collected and frozen for future use.
After confirmation of an antigenic response, serum was collected and frozen for further use.
After confirmation, infected tissue culture fluid (ITCF) was collected and stored at -70°C until further processing.
For children seen only in the second month after measles infection a blood sample was collected for possible confirmation with an IgM antibody test.
For the final time point, blood was collected after histological confirmation of recurrent disease or when patients returned for another clinical review and did not have any clinical evidence of recurrent disease.
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CEO of Professional Science Editing for Scientists @ prosciediting.com