Sentence examples for confirmation of transcript from inspiring English sources

For a unique specific target or a small set of specific targets, confirmation of transcript variation by further evaluation of protein variation by either immunohistochemistry or western blot will undoubtedly validate the initial choice of the best HKG.

Confirmation of transcript boundaries was a prerequisite to studies of gene function because approximately a third of the NBS-LRR-encoding and related genes in the public database were previously annotated incorrectly and therefore had to be corrected by manual re-annotation [ 7].

Confirmation of FVIII transcript identity was obtained by Blastn (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi

For the confirmation of hybrid transcript candidate, we aligned our candidate sequences to genomic DNA sequence by using the SIM4 program [ 24] and the alignment around the fusion point was manually inspected.

The high rate of successful confirmation of real transcripts indicates the reliability of ARTADE2 for predicting novel genes.

Therefore, we have not pursued any further confirmation of single transcripts differential regulation at RNA or protein levels.

In this regard, mass spectrometry is a powerful tool that can contribute to the identification of novel genes and assist in confirmation of predicted transcripts as well as correction of incorrect assignments from automated gene annotations [ 3].

In the characterization of the σ regulon of Salmonella, determination of the genomic binding sites for the Eσ allows confirmation of primary transcripts indicated by microarray analysis and recognition of potential σ-regulated genes that might not have been detected due to instability of the transcripts.

Some of these mention transcript-specific analysis as a possible application, however, a confirmation of a predicted transcript expression by independent means such as real-time RT-PCR has not yet been reported.

For the confirmation of a hybrid transcript candidate, we inspected whether the fusion point corresponded to a pair of known splice sites.

Here we report the computational detection of an interchromosomal RNA fusion between ZC3HAV1L and CHMP1A from RNA-seq data of normal human mammary epithelial cells, and experimental confirmation of the chimeric transcript in multiple human cells and tissues.