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Collected samples and potential contaminants should also be investigated both before and after irradiation by ultraviolet rays or electron beam for further confirmation of sample origin.
A method for post hoc confirmation of sample identity is therefore highly desirable.
After confirmation of sample quality as described above, RNA samples were hybridized using a human U133A Microarray Chip.
Total RNA sample aliquots were frozen in liquid nitrogen and shipped on dry ice to the Beijing Genomics Institute (BGI) in Hong Kong or delivered to the Genome Sequencing Analysis Facility (GSAF) at UT. Confirmation of sample quality and concentration was conducted at each facility using the Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara CA, USA).
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The device was further modified to enable visual confirmation of the sample collection and to reduce the sample volume size [ 19].
Diagnosis of VAP is based on clinical suspicion and microbiologic confirmation of a sample obtained from the lower respiratory tract.
Two-tailed Pearson's statistics was used for correlated expression of CD68 and CD163, with SOX2, CD44, and ALDH1 after confirmation of the sample with Gaussian distribution.
For research and confirmation of suspected samples, some labs are also using IP (immunoprecipitation) and Western blot assays with cell lysates (HeLa, HEpG2, HEp-2, Jurkat or PC3 cell lines) known to express ample levels of LEDGF/DFS70 protein [25, 28, 29].
Confirmation of aberrant samples was done by Sanger sequencing [ 8].
Confirmation of aberrant samples was done by Sanger sequencing (van der Hout et al, 2006).
For confirmation of positive samples, a flaB gene nested PCR and sequencing of amplicons were performed as described (9 ).
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