The decision ultimately rests with the confirmation candidate, but the child's adult patron, priest, parents and siblings can provide important insights.
Sanger sequencing using standard polymerase chain reaction (PCR) amplification procedures was performed for confirmation of candidate variants seen in WES as well as segregation of candidate variants within the family.
After in vitro screening and sequencing confirmation, 3 candidate peptides BP1, BP2 and BP3 were selected for further analysis and were synthesized in the peptide synthesis facility of the Academy of Military Medical Sciences, China.
As a verification step and to provide size and sequence confirmation of candidate miRNAs, total RNA or LMW RNAs extracted from a mixture of male and female S. japonicum adult worms were separated by electrophoresis on a 15% polyacrylamide gel under denaturing conditions, and transferred to Hybond-N nylon membrane (Pall).
However, this hypothesis requires further confirmation and candidate gene complementation tests.
FZ performed the microarray data analysis, and LH carried out the qRT-PCR confirmation of candidate genes.
You'll also need your exam confirmation number, Candidate Examination Document, two pieces of signature-bearing I.D. (one with a photo), and an ordinary (non-solar) calculator.
The second mapping stage comprised candidate locus confirmation and prioritization of candidate genes.
For President Bush and Senate Republicans, the most concrete satisfaction in winning control of the Senate may come in early confirmation of several candidates for the federal bench whose nominations were blocked or defeated by Democrats who said they were too conservative.
This particular candidates confirmation would have needed to pass a high, er, bar.
