The reason for requiring multiple clinic visits for identification as a case is that insulin-requiring diabetes was occasionally recorded as a tentative diagnosis explaining a clinic visit pending confirmation by laboratory data.
However, we now demonstrate according to the expression data provided by TCGA in confirmation of data published by Haferlach et al, that these genes do not show any expression or very low expression in blood or bone marrow cells of AML patients [ 26].
Thus, although we lack confirmation by quantitative expression data, it appears that the two GM lines Pm3b#2 and #4, where the transgene showed the strongest phenotypic effects, also had the strongest transgene expression.
After confirmation by qPCR tests (data not shown) the FN library was not considered further, reducing the assembly to 241,870 reads in 38,415 contigs and 2,761 singletons.
This will need confirmation from data generated by other publication groups and further research.
Further confirmation was provided by data-dependent MS/MS experiments for structural elucidation.
Confirmation of these data by other studies is required before resultant implications are considered.
Due to the limited amount of total RNA, the confirmation of expression data by quantitative real-time PCR was not possible.
Eight genes were selected for the confirmation of DEG data by real-time PCR, using the SYBR Premix Ex Taq kit (Takara, Japan) according to the manufacturer's instructions in a StepOne machine (Applied Biosystems, Carlsbad, CA).
Confirmation of these data by a more quantitative technique will be essential to determine more precisely the relative expression of this gene set in patients with SLE, as well as those with other autoimmune diseases.
By way of confirmation of our data there are experimental studies that confirm the development of a pulmonary inflammatory process following exposure to fiberglass.
