All annotations were confirmed by manual inspection, including confirmation of active residues and phylogenetic tree analyses.
We cannot confirm that all patients treated for TB without microbiologic confirmation had active disease because they were ineligible for our vaccine study and did not have follow-up to assess response to therapy.
Seven were ineligible: four had normal markers with no biopsy confirmation of active cancer, one had brain metastases, one was treated as adjuvant following complete resection of active cancer and one was registered too late (during the third cycle of chemotherapy)).
A core element associated with a rumor is lack of verification, and some rumors that take time to identify, such as those involving consideration or confirmation, require active effort to discern between true and false and one needs to determine whether there is propagation or not.
Confirmation of active site annotation across species was achieved by consulting relevant databases.
For confirmation, 55 active and 5 inactive retest compounds were chosen for testing as independent samples, and in this confirmation 90% of originally active compounds were reconfirmed as active (Supplementary Table 3).
Thus, the confirmation of active components in HMs is of great significance to ensure their quality and curative effects and also to elucidate their complicated pharmacodynamic mechanisms.
Confirmation of active infection consisted of amplification of B. miyamotoi DNA/RNA in blood by species-specific PCR and detection of anti-borreliae immunoglobulin (Ig) M in acute- and/or convalescent-phase serum samples.
Nevertheless, it should be mentioned that PCR has a limited sensitivity, particularly in AFB smear negative specimens, and that TB culture of clinical specimens remains the gold standard for the confirmation of active TB infection and the assessment of mycobacterial resistance [ 16].
The proposed smart cantilever beam with the prescribed repeatable and controllable disturbances serves as a very suitable model in laboratory confirmations for active controller designs.
