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The collected data were statistically confirmed using analysis of variance (F Test) at P ≤ 0.05 and the differences between each treatment were determined by Tukey test (P ≤ 0.05) using Minitab Version 14.12.

The model adequacy was confirmed using analysis of variance (ANOVA).

These results were confirmed using analysis of variance (ANOVA) with Tukey honest significant difference (HSD).

Statistically significant alterations were identified using multivariate statistics, including principal components analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA) and further confirmed using analysis of variance (ANOVA).

Finally, overlap of development-related response between the different datasets was confirmed using analysis by ANOVA in each separate dataset (Additional file 2: Figure S1 E-H & Additional file 1: Table S1).

Findings from the univariate analysis were confirmed using multivariate analysis to find independently significant predictors of young people being detected by CSP rather than GUM (table 2).

Moreover, the formation of multi-walled carbon nanotubes (MWCNTs) was confirmed using thermogravimetric analysis (TGA).

Also, chemical structure of the produced biosurfactant was confirmed using FTIR analysis.

Moderate thermal stability enhancement of PA 6 after adding 30B and/or POEMA was confirmed using thermogravimetric analysis (TGA).

Further, the synthesized silver nanoparticles were confirmed using XRD analysis.

The crystalline nature of the as-synthesized Pt@Cu nanostructures was further confirmed using XRD analysis.

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