Exact(32)
"However, these were followed by studies that could not convincingly confirm this interaction or that did not find an interaction at all".
We could not confirm this interaction by co-immunoprecipitation assays (not shown).
To further confirm this interaction, co-immunoprecipitation experiments of endogenous SR proteins were also carried out (Figure 2B).
To confirm this interaction using exogenous HSPA8, we transfected HA-tagged HSPA8 with OmiS306C/G399S over-expression or the empty vector (BH4).
To further confirm this interaction in vivo, U2OS cells were transiently transfected with HA-MAD2B and FLAG-tagged CLTA (FLAG-CLTA) expression constructs.
To further confirm this interaction between LdUba5 and LdUfm1, we expressed wild type and mutant forms of LdUfm1 and performed coimmunoprecipitation experiments (Figure 1E).
Similar(28)
We have identified RanBPM as a potential interacting protein and confirmed this interaction by in vitro assay.
We have identified RanBPM as an interacting protein by the yeast two-hybrid assay and confirmed this interaction in-vitro by co-immunoprecipitation.
ANOVA plot confirmed this interaction, showing a higher effect of exercise in YTG than in MTG (Supplementary Fig. 1).
In addition, a SUZ12 ChIP followed by telomeric dot-blot confirmed this interaction of SUZ12 with telomeric DNA, which was decreased upon downregulation of SUZ12 levels by shRNA (Fig. 4c).
We confirmed this interaction by also testing the pools of endogenous proteins in these cells.
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