This study confirmed the locus of LDI gene and detected a DArT marker, bpb-0068, associated with LDI activity.
The current results confirmed the locus of the gene controlling LDI activity and identified a new DArT markers associated with LDI activity.
Interestingly, association to the same SNP and a second, independently associated polymorphism in the region (rs10499194) was detected in a GWAS in a US population, confirming the locus as important in RA causation (5).
Further fine mapping with an extended pedigree, with additional 10 markers, confirmed the locus at 9p21.2-p22.3 with a multipoint LOD = 4.21 within the interval between D9S1870 and D9S161 (Supplementary Fig. 1).
In addition, three significant SNPs for colour a* of LM were identified in a region around 58.13 Mb on SSC12, which confirms the locus reported by Luo et al. [ 39] for a Large White × Minzhu F2 intercross population.
More recently, a functional variant in DNASE1L3 was implicated in a familial form of SLE [ 33], and a pan-meta-analysis of SLE and SSc also confirmed the locus near PXK, but in particular for ACA-positive SSc (rs2176082 [ 7]; P = 1.4 × 10−4 in our data) strengthening the evidence that this locus plays a role in both diseases.
Likewise, the ΔyscN mutant strain was screened on Congo Red [57] agar plates to confirm the pgm locus was intact.
To confirm the correct locus had been identified, Syx5AR113/CyO Drosophila were screened for copper tolerance (Fig. 1).
We confirm the SLC17A1 locus as the third associated with gout at a genome-wide level of significance.
