Sentence examples for confirm targets from inspiring English sources

To confirm targets identified by ChIP-seq, all ChIP assays were performed using StaphA cells.

Potential targeted clones were individually grown and rescreened by Southern blotting to confirm targeting.

To confirm target engagement of the mTORC1/4E-BP1 axis, 4E-BP1 phosphorylation was measured in tissue slice homogenates following exposure to CZ415 (1 μM).

Medicinal chemists also study compound biodistribution as an in vivo screen following in vitro tests (which e.g. confirm target binding).

In our second approach we used knock-in reporter cell lines to confirm target gene activation by Notch1.

A luciferase reporter assay was conducted to confirm target association.

Real-time PCR was used to confirm target gene expression changes.

Fluorescence microscopy was performed to confirm target-specific localization of Pan-IR700.

To confirm target enrichment, each PCR product was evaluated first by standard end point PCR.

RT-PCR and/or western blot analyses were performed to confirm target knockdown by siRNA.

In order to confirm target specificity we cloned and sequenced amplified regions (ABI 3130xl, Applied Biosystems).