Sentence examples for confirm target from inspiring English sources

To confirm target engagement of the mTORC1/4E-BP1 axis, 4E-BP1 phosphorylation was measured in tissue slice homogenates following exposure to CZ415 (1 μM).

Medicinal chemists also study compound biodistribution as an in vivo screen following in vitro tests (which e.g. confirm target binding).

In our second approach we used knock-in reporter cell lines to confirm target gene activation by Notch1.

A luciferase reporter assay was conducted to confirm target association.

In order to confirm target specificity we cloned and sequenced amplified regions (ABI 3130xl, Applied Biosystems).

RT-PCR and/or western blot analyses were performed to confirm target knockdown by siRNA.

Potential targeted clones were individually grown and rescreened by Southern blotting to confirm targeting.

In order to confirm target-specific demethylation noted in primary tumors, we devised a rapid, quantitative assay for specifically measuring non-methylated promoters, which we termed Quantitative Unmethylation-Specific PCR (QUMSP).

Fluorescence microscopy was performed to confirm target-specific localization of Pan-IR700.

SLC38A1, SLC38A2, SLC38A4 and β-actin mRNA expression was analysed by quantitative PCR to confirm target-specific knockdown.

To confirm targets identified by ChIP-seq, all ChIP assays were performed using StaphA cells.