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To further confirm specificity of the TBC1D4 phospho-specific antibodies, we performed immunodepletion analyses using TBC1D1 and TBC1D4 antibodies and muscle lysates from human skeletal muscle.
The optimized assay condition was validated according to ICH guidelines to confirm specificity, linearity, accuracy and precision.
Adequate controls, including vehicle and similarly modified missense or scrambled sequences, are essential to confirm specificity and to exclude toxicity.
Melting curve analysis was used to confirm specificity of amplification.
Controls were included to confirm specificity and validity.
To confirm specificity, mitochondria were also isolated from nNOS-deficient muscle directly electroporated with nNOS siRNA.
The kinase-dead variant K1906M served as negative control to confirm specificity of the observed signals.
To detect direct binding between NM23 and MUC1*1110-ecd peptides and to confirm specificity, another nanoparticle experiment was performed.
All real-time PCR assays used in this study were validated and optimised to confirm specificity and sensitivity.
To confirm specificity of TLR-mediated responses, HEK cell lines were stimulated with either a TLR4 or TLR2 agonist.
These data confirm specificity of BSO treatment and demonstrate that glutathione depletion increases oxidative stress in adipocytes and inhibits adipogenesis.
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